Hygromycin B-resistance phenotype acquired inParacoccidioides brasiliensisvia plasmid DNA integration
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چکیده
منابع مشابه
Enzymatic Basis of Hygromycin B Resistance in Escherichia coli
A plasmid conferring resistance to the aminocyclitol antibiotic hygromycin B was isolated from Escherichia coli. The gene conferring resistance to this drug was cloned in pBR322, and the gene was localized to a fragment of ca. 1,510 base pairs. Resistance to hygromycin B is determined by an aminocyclitol phosphotransferase that modifies hygromycin B and structurally related antibiotics. The spe...
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This study provides evidence of direct transfer of functional DNA from species to species, specifically Escherichia coli pGFPuv and Enterobacter aerogenes. An Escherichia coli made pGFPuv invasive by transformation via miniprep transfers DNA after simple co-incubation with Enterobacter aerogenes. Transfer efficiency was enhanced or disturbed in the bacteria by altering pH. Expression of the acq...
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∗ Corresponding author Albino strain KB20M1 of Monascus purpureus was genetically transformed to hygromycin B resistance with cosmid pMOcosX, using biolistic bombardment. Conidia and mycelial fragments were used as the recipient material and rupture disk strengths of 6.2 and 9.3 MPa each yielded a transformed isolate from five bombardments. Southern analysis suggested a single copy of the cosmi...
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Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus. Ribosomes from this Gram-positive mycelial bacterium are inhibited in vitro by the antibiotic. In contrast, the streptomycete is highly resistant to the drug in vivo since it possesses hygromycin B phosphotransferase activity. This enzyme has been shown by g...
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Campylobacter jejuni genetic manipulation is restricted by the limited number of antibiotic resistance cassettes available for use in this diarrheal pathogen. In this study, two antibiotic resistance cassettes were developed, encoding for hygromycin B and apramycin resistance, for use in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. First, the mark...
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ژورنال
عنوان ژورنال: Medical Mycology
سال: 2005
ISSN: 1369-3786,1460-2709
DOI: 10.1080/13693780500159118